Extended Data Fig. 8: Cell-cell communication analysis revealing ligand-receptor interaction at the myelin-axon interface. | Nature Neuroscience

Extended Data Fig. 8: Cell-cell communication analysis revealing ligand-receptor interaction at the myelin-axon interface.

From: Myelin–axon interface vulnerability in Alzheimer’s disease revealed by subcellular proteomics and imaging of human and mouse brain

Extended Data Fig. 8: Cell-cell communication analysis revealing ligand-receptor interaction at the myelin-axon interface.

a-b. (A) Cell clustering and (B) cell type annotation of snRNAseq data from AD human frontal cortex (Braak stage 6) and controls (Braak stage 0). c. Enrichment analysis shows that myelin-axon interface proteomics (MAG or CASPR-labeled) are highly enriched in neurons and oligodendrocytes, but not in other cell types (related to Fig. 5b). Each row depicts contingency tables for each hypergeometric test (from top to bottom: p-values: 0.0005275, 0.0007784, 0.009328, 0.0001295). In these rows, values with two decimal places indicate residuals and the size of the circles; positive residuals denote that the observed values were more frequent than expected, while negative residuals indicate lower-than-expected frequencies. Quantifications were performed two-sided. d-e. Violin plots showing RNA expression levesl of ligand-receptor pairs in (D) control human postmortem brains (Braak stage 0) and (E) AD human postmortem brains (Braak stage 6).

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