Extended Data Fig. 9: Paranode-enriched and myelin-axon interface Alzheimer’s disease proteomes reveal unique subcellular changes not observed by bulk proteomics or single cell RNA transcriptomics.

Heatmaps display (a) Paranode Alzheimer’s-associated proteomes (PAPs) and (b) Myelin-axon interface Alzheimer’s proteomes (MAPs). Heatmap denotes log₁₀ (spectral counts). Comparison between PAPs or MAPs and bulk proteomics data (middle panel, Johnson et al.¹⁶), or single nuclei RNA sequencing transcriptomics (right panels, Mathys et al.¹¹) were performed. Both bulk proteomics and snRNAseq data were obtained from their original studies. Neuronal cell types (yellow box) and oligodendrocyte/OPC (green box) were highlighted in the snRNAseq data. Abbreviations: FC = fold change; DEG = differentially expressed genes. (A and B) Quantifications of subcellular proteomic data derived from this study were performed two-sided.