Extended Data Fig. 6: Additional data to confirm CLOCK KO reduces dendritic complexity and spine density in iPSC-derived neurons.
Date collected from different CLOCK KO cell lines (KO#2 and KO#7) at different differentiation stages (Day 21, Day 28, and Day 35). Data of WT and KO#2 at Day 28 are replotted from Fig. 7, and date of Day 21 are replotted from Extended Data Fig. 12. (a) Total length of dendrites normalized to number of neurons. (b) Number of segments of dendrites per neuron. (c) Number of puncta per µm of dendrites. For all panels, each data point is an independent culture of neurons on a circle glass cover slip sitting on the bottom of a well in a 24-well plate. We did GLM with genotype/treatment as fixed factors with Tukey’s test for post-hoc analysis. Details of statistical results can be found in Supplementary Table 1. All data are shown as means ± SEM. All statistics were two-sided tests. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
