Extended Data Fig. 2: Penetrating arteriole and arteriole-capillary transition zones of adult and aged mice are similar in diameter.
a. Maximally projected image in layer 6/CC showing a top-down view of the vascular bed surrounding the main trunk of a PCV in an adult mouse. Red arrow marks a penetrating arteriole trunk. b. Inset showing a magnified view around the penetrating arteriole with the first three branch orders of vessel offshoots, referred to as arteriole-capillary transition zone. c. Average diameter of the penetrating arteriole trunk throughout cortical depth in adult and aged mice. Each dot corresponds to an average diameter of arteriole trunks at the corresponding depth in a single animal. One-way ANOVA with Holm-Sidak post-hoc test. Adult, n = 12 mice, with 3-4 vessels per cortical depth per animal; Aged, n = 11 mice, with 3-4 vessels per cortical depth per animal. Data shown as mean ± SD. d-f. Average diameter of vessels in the arteriole-capillary transition zone of adult and aged mice in Layer 2/3 (d), Layer 4 (e), Layer 6/CC (f). Each dot corresponds to an average diameter of vessels of the corresponding branch order in a single animal. One-way ANOVA with Holm-Sidak post-hoc test. Adult, n = 12 mice (n = 11 for Layer 6/CC); Aged, n = 11 mice. Data shown as mean ± SD.
