Fig. 6: In vivo ablation of pre-convergence capillaries leads to broad vasoconstriction and hypoperfusion with worsened outcomes in layer 6/CC. | Nature Neuroscience

Fig. 6: In vivo ablation of pre-convergence capillaries leads to broad vasoconstriction and hypoperfusion with worsened outcomes in layer 6/CC.

From: Impaired capillary–venous drainage contributes to gliosis and demyelination in mouse white matter during aging

Fig. 6: In vivo ablation of pre-convergence capillaries leads to broad vasoconstriction and hypoperfusion with worsened outcomes in layer 6/CC.The alternative text for this image may have been generated using AI.

a,b, Laser-induced ablation of pre-convergence capillaries in layer 6/CC and off-target sham controls. The insets show changes evoked in a neighboring capillary before (a) and 21 d after irradiation (b). c,d, Vascular network schematics showing blood flow changes in a (c) and b (d). e,f, RBC flux of neighboring pre-convergence capillaries before and 21 d after capillary ablation in layer 4 (e, left) and layer 6/CC (e, right), or after sham irradiation (f) (layer 4, n = 40 vessels from 8 ROIs; layer 6/CC, n = 48 vessels from 9 ROIs; sham irradiation, n = 24 vessels from 4 ROIs). The data are shown as the mean ± s.e.m. Two-sided, paired Student’s t-test: layer 4, ***P = 0.0003; layer 6/CC, ****P < 0.0001; sham, P = 0.2998. g, Percentage change from baseline for pre-convergence capillary RBC flux at 21 d after laser irradiation. The data are shown as the mean ± s.d. One-way ANOVA with Dunnett’s post hoc comparison tests: *P < 0.05. h, Proportion of total capillaries with no flow at 21 d after ablation. i,j, Pre-convergence capillary diameter before and 21 d after capillary ablation in layer 4 (left) and layer 6/CC (right) (i) or after sham irradiation (j) (layer 4, n = 40 vessels from 8 ROIs; layer 6/CC, n = 45 vessels from 9 ROIs; sham irradiation, n = 24 vessels from 4 ROIs). The data are shown as the mean ± s.e.m. Two-sided, paired Student’s t-test: layer 4, ***P = 0.0003; layer 6/CC, ****P = 0.000013; sham, P = 0.8694. k, Percentage change from baseline for pre-convergence capillary diameter at 21 d after laser irradiation. The data are shown as the mean ± s.d. One-way ANOVA with Tukey’s post hoc comparison tests: *P < 0.05. l, Change in RBC flux as a function of change in diameter across all capillaries examined. The shaded area represents the 95% confidence bands of the best-fit line. For all experiments, n = 5 adult mice. See Supplementary Table 2 for details of statistics.

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