Extended Data Fig. 2: Replication and validation of cytoplasmic NRE-capture-seq. | Nature Neuroscience

Extended Data Fig. 2: Replication and validation of cytoplasmic NRE-capture-seq.

From: Aberrant splicing exonizes C9orf72 repeat expansion in ALS/FTD

Extended Data Fig. 2

(a) Read coverages of FB503 and FB506 cytoplasmic RNA inputs (top two tracks) and NRE-captured RNAs (bottom two tracks). (b) Schematics of RT-qPCR primers used in (c) and (d). (c) RT-qPCR quantification of the enrichment of aberrant Ex1c-Ex2 splice junction by NRE-capture, comparing C9 NRE+ and NRE− samples. (d) RT-qPCR quantification of siRNA knockdown efficiency in C9 NRE+ fibroblasts. siNT, non-targeting siRNA. siEx2 1 and 2, two siRNAs targeting C9orF72 exon 2. Data represent mean ± s.d. n = 2 biological replicates.

Back to article page