Extended Data Fig. 2: Distinct MS subpopulations are activated during dehydration and satiation.
From: A bottom-up septal inhibitory circuit mediates anticipatory control of drinking
a, Timeline for dual labeling of dehydration-activated (FosTrap) and satiation-activated (c-Fos) neurons in Fos-CreERT2 mice. b, Representative MS histology showing dehydration-activated (red), satiation-activated (green), and overlapping neurons (n = 5 mice). Scale bar, Scale bars, 200 µm in the MS, 50 µm for the magnified images. c, Quantification of overlap across satiation-activated and dehydration-activated neurons. d-e, Dehydration-responded (d) and rehydration-responded (e) neuronal populations project to the SFO but not the MnPO (n = 5 mice). Scale bars, 200 μm in the MS and MnPO, 100 μm in the SFO.
