Extended Data Fig. 7: Extended clustering and quality control in the astrocytic subset.
From: C9orf72 hexanucleotide repeat expansions impair microglial response in ALS
(a) UMAP plot visualizing 10,119 nuclei from the motor cortex and 13,364 nuclei from the spinal cord. Cell populations are coloured based on their assigned cluster into astrocytes, oligodendrocytes, doublets/low quality. (b) Heatmap showing the top 20 most expressed genes per cluster. (c) Heatmap showing average expression of the top 100 markers from the original 8 astrocyte clusters (Fig. 5a) onto the novel clustering after integration with Gerrits et al.22. (Fig. 5b). (d) Proportion of cells per cluster (y-axis) for each individual, across all the different anatomical regions. (e) Heatmaps showing differentially expressed genes (log2FC, p-value adjusted <0.05) between C9-ALS or sALS and healthy controls, and displayed for the spinal cord, motor cortex or both locations combined.
