Extended Data Fig. 10: Synaptic phagocytosis. | Nature Neuroscience

Extended Data Fig. 10: Synaptic phagocytosis.

From: Astrocytic Sox9 overexpression in Alzheimer’s disease mouse models promotes Aβ plaque phagocytosis and preserves cognitive function

Extended Data Fig. 10

a, c, e. Representative confocal Z-stack images of GFP-positive astrocytes (blue) co-stained with CathD (green) and vGlut1 (red) in the APPNLGF; Sox9Con and APPNLGF; Sox9cKO at 4 months (a), AAV-con and AAV-Sox9ov in APPNLGF at 8 months (c), and AAV-con and AAV-Megf10ov in APPNLGF at 8 months (e). Scale bars, 5 µm. Quantification of phagocytic index for engulfed Aβ normalized by astrocyte cell body area. n = 5 mice for each group (right panel). b, d, f. Representative confocal Z-stack images of GFP-positive astrocytes (blue) co-stained with CathD (green) and PSD95 (red) in the APPNLGF; Sox9Con and APPNLGF; Sox9cKO at 4 months (b), AAV-con and AAV-Sox9ov in APPNLGF at 8 months (d), and AAV-con and AAV-MEGF10ov in APPNLGF at 8 months (f). Scale bars, 5 µm. Quantification of phagocytic index for engulfed Aβ normalized by astrocyte cell body area. n = 5 mice for each group (right panel). g. Schematic illustration for the AAV-hSyn-PSD-mCherry-pHluorin system. h-l. Representative confocal Z-stack images of GFP-positive astrocytes (blue) co-stained with mCherry (red) and pHluorin (green) in the WT (h), AAV-con and AAV-Sox9ov in APPNLGF at 8 months (i), and AAV-con and AAV-MEGF10ov in APPNLGF at 8 months (k). The yellow arrow shows mCherry only in astrocytes. Scale bars, 5 µm. Quantification of phagocytic index for engulfed Aβ normalized by astrocyte cell body area (j, l). n = 5 mice for each group. A two-sided unpaired Student’s t-test (a-l) was used for analysis. The mean ± s.e.m. All illustrations were created using BioRender.com.

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