Extended Data Fig. 2: Altered astrocyte morphology in the aging brain.
a. UMAP plot of astrocyte clusters from the whole brain of 2-3 month-old mice and 21-23 month-old mice. b. Violin plot of Aldh1l1 expression in each group. c. Count value of Aldh1l1 RNA expression from RNAseq of hippocampal astrocytes of 4 month-old mice and 18 month-old mice. n = 3 mice for each group d. Representative confocal Z-stack images of GFP in the hippocampus of 4 month-old mice and 18 month-old mice. Scale bars, 20 µm. e. Quantification of the number of interactions by Sholl analysis of hippocampal astrocytes of 4 month-old mice and 18 month-old mice. n = 6 mice for each group. f. Schematic illustration for WT; Aldh1l1-creER; LSL; tdTomato mouse. g. Representative confocal Z-stack images of tdTomato-astrocytes in the hippocampus of 4 month-old mice and 18 month-old mice. Scale bars, 20 µm. h. Quantification of the number of interactions by Sholl analysis of hippocampal tdTomato-astrocytes of 4 month-old mice (n = 13 mice) and 18 month-old mice (n = 14 mice). i. UMAP plot of total cell clusters from the whole brain of 2-3 month-old mice and 21-23 month-old mice. j, k. Atp1b2 (ACSA-2 antigen) expression (j) and Aldh1l1 (k) from the total cell clusters at 2-3 months and 21-23 months. l. Representative confocal Z-stack images of Sox9 (red) and GFP (green) in the hippocampus of 4 month-old mice and 18 month-old mice. Scale bars, 100 µm. m. Percentage of Aldh1l1-expressed astrocytes in the total Sox9-expressed cells. n = 6 mice for each group. Two-sided unpaired Student’s t-test (c) and Two-way ANOVA with Sidak’s correction (e, h) were used for analysis. The mean ± s.e.m. ns: not significant. All illustrations were created using BioRender.com.
