Extended Data Fig. 8: Lim1 does not regulate entrainment of the clock phase to temperature cycles.
From: Behavioral adaptation to warm conditions via Lim1-mediated acceleration of neuronal clocks
Circadian clocks can be reset not only by light-dark cycles, but also by temperature cycles with an amplitude as low as ~2 °C61. DN1ps mediate entrainment to low-amplitude temperature cycles31; we asked whether this requires Lim1. Double-plotted actograms of control (DN1-Gal4) and Lim1-depleted (DN1>lim1-RNAi) flies. a, Flies were entrained to 12-h light/12-h dark (LD) cycles for 3 days before shifting to the first temperature cycle (TC1, 21 °C/25 °C, constant darkness, 6 h delayed relative to the LD cycle) for 5 days, followed by 5 days in the second temperature cycle (TC2, 21 °C/25 °C, constant darkness, 6 h delayed relative to TC1), finishing with 5 days in constant temperature and darkness. n = 31 and 30 for DN1-Gal4 and DN1>lim1-RNAi, respectively, Student’s t-tests. b, As in a, but with TC1 and TC2 at 25 °C/29 °C. n = 31 and 31 for DN1-Gal4 and DN1>lim1-RNAi, respectively, Student’s t-tests. c, Flies were entrained to LD cycle at 25 °C for 3 days before shifting to the first temperature cycle (TC, 25 °C/27 °C, constant darkness, 8 h advanced relative to the LD cycle) for 6 days, finishing with 8 days in constant temperature and darkness. n = 28 and 31 for DN1-Gal4 and DN1>lim1-RNAi, respectively, Student’s t-tests. d, Flies were kept in constant light (LL) for 3 days before shifting to the first temperature cycle (TC1, 25 °C/29 °C, constant light, 8 h advanced relative to the LD cycle) for 6 days, followed by 8 days in the second temperature cycle (TC2, 25 °C/29 °C, constant light, 6 h delayed relative to TC1). n = 28 and 30 for DN1-Gal4 and DN1>lim1-RNAi, respectively, Student’s t-tests. Quantification represents mean ± s.e.m.
