Extended Data Fig. 7: e-SP-mediated AEA is independent of PTX-insensitive G protein coupled receptor.
(a)Time course of the synaptic efficacy before and after AEA puff (red arrow) in PTX treated slices. (b) Relative changes from control basal values of the synaptic efficacy before (grey bar) and after (red bar) AEA puff (n = 6/2, p < 0.01). (c)Time course of the heteroneuron EPSC amplitude before and after ND (red arrow) in control condition of PTX treated slices. (d) Relative changes from control basal values of heteroneuron EPSC amplitude before (grey bars) and after ND (red bars) in control conditions (n = 5/3, p < 0.05) and in the presence of LEI-401 (n = 5/3, p = 0.2). (e) Schematic representation of the experimental design of ATP puff to astrocytes expressing GCaMP6f in PTX treated slices. (f) Left. Pseudocolor images showing astrocytes calcium elevation in basal condition and in response to ATP puff. Right. Representative traces showing astrocytes calcium elevation to ATP puff. Scale bars 2 ΔF/F, 10 s. (g) Heat map of Calcium-based fluorescence levels showing astrocyte Calcium events to ATP puff (red arrow, time 0), in control condition (18 ROIs). (h) Relative astrocyte calcium event probability before (grey bar; basal) and after ATP puff (red bar; ATP puff) in astrocytes expressing GCaMP6f and PTX treated slices (n = 8/2, p < 0.01) and in the absence of Gq-DREADDs (n = 8, **p < 0.01;). Adult animals ( ≥ 6 weeks; males and females) were used; *p < 0.05, **p < 0.01, ***p < 0.001; Student’s paired and unpaired t-test. Data are mean ± s.e.m.
