Extended Data Fig. 5: 2-AG, but not AEA, modulates inhibitory synaptic transmission.
(a) Drawing showing the hippocampus location in slices. (b) Top. IPSCs traces recorded in the presence of CNQX 20 µM + D-AP5 50 µM, showing IPSC amplitudes in basal (black trace), and after ND in control (red trace), in the presence of LEI-401 (blue trace) and in THL (blue trace). (Scale bars: 10 pA, 10 ms). Bottom. Time course of IPSC amplitude before and after ND (pink arrows) in control condition, in the presence of LEI-401 and THL. (c) Relative changes of IPSC amplitude before and after ND in control conditions (n = 9/5, p < 0.01), LEI-401 (n = 8/4, p < 0.01) and THL (n = 6/2, p = 0.9). The decrease in the synaptic efficacy was preserved in LEI-401 (n = 8, p < 0.05) but abolished in THL (n = 6, p = 0.9); one-way ANOVA, post hoc Holm–Sidak. (d) Traces showing IPSC amplitudes in basal (1; black trace), and after AEA puff (2; red trace). (Scale bars: 20 pA, 10 ms). (e) Time course of the IPSC amplitude before and after bath applied AEA (3 µM; green bar). (f) Relative changes of IPSC amplitude before (1) and after (2) bath applied AEA (3 µM; n = 6/2, p < 0.01). Adult animals ( ≥ 6 weeks; males and females) were used; Student’s paired t-test. #p < 0.01, ##p < 0.01, ###p < 0.001, nonsignificant (p > 0.05). Data are mean ± s.e.m.
