Extended Data Fig. 2: Biophysical properties of human pyramidal neurons in high- vs low-grade glioma from individual patients.

Electrophysiological recordings were averaged and assessed according to individual patients diagnosed with high-grade (n = 32) and low-grade (n = 16) glioma. Trends in Rheobase, Maximum Action Potential (AP) Rate of Rise, AP amplitude and Resting Membrane Potential (RMP) were similar to the analysis performed in individual recordings. a, AP Firing Rate of pyramidal neurons from high- and low-grade glioma in response to somatic current step injections (20 pA, 1200 ms; n = 32 (high), 16 (low) patients). b, Rheobase in pyramidal neurons from high- and low-grade glioma (n = 32 (high), 16 (low) patients; p = 0.015, Mann-Whitney test). c, Maximum AP Rate of Rise (dV/dT) in pyramidal neurons recorded from high- and low-grade glioma (n = 32 (high), 16 (low) patients; p = 0.02, Mann-Whitney test). d, AP phase plot of membrane potential relative to threshold in high- and low-grade glioma (n = 32 (high), 16 (low) patients). e, Peak AP Amplitude in pyramidal neurons in high- and low-grade glioma (n = 32 (high), 16 (low) patients; p = 0.017, Mann-Whitney test). f, RMP in pyramidal neurons from high- and low-grade glioma (n = 32 (high), 16 (low) patients; p = 0.474, Mann-Whitney test). Data are presented as mean values ± SEM. ns, p > 0.05; *, p < 0.05.