Extended Data Fig. 5: Morphological reconstruction of high- and low-grade neurons. | Nature Neuroscience

Extended Data Fig. 5: Morphological reconstruction of high- and low-grade neurons.

From: Increased neural excitability and glioma synaptic activity drives glioma proliferation in human cortex

Extended Data Fig. 5

Pyramidal neurons (average somatic depth below pia, 381 ± 21 μm) were filled with the fluorescence marker biocytin-TMR via the patch pipette. Neuron morphology was imaged using confocal microscopy and reconstructed before Sholl analysis was performed. (top) Neuron morphology and (bottom) Number of intersections versus Sholl radius for pyramidal neurons within (a) high-grade and (b) low-grade tissue. c, Comparison between dendritic length and distance from soma for neurons in high-grade (blue; n = 8 cells) ad low-grade (green; n = 6 cells) glioma (p = 0.498, Komogorov-Smirnov test). d, Differences in total length (top; p = 0.852, Mann Whitney test) and intersections (bottom; p = 0.883, Mann Whitney test) for neurons in high-grade (blue; n = 8 cells) and low-grade (green; n = 6 cells) glioma. Data are presented as mean values ± SEM. ns, p > 0.05.

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