Extended Data Fig. 2: Spatiotemporal alterations in TGF-β pathway genes in the spinal cord.
From: TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration
a Gene Ontology (GO) enrichment analyses of the DEGs from NanoString nCounter analysis between YM (2-3 months) and OM (12 months) reveal the Top 10 enriched Biological Processes (BP), Cellular Components (CC), and Molecular Functions (MF), while WikiPathway analysis highlighted all the enriched pathways. The bar charts represent the False Discovery Rate (FDR) in -log10 scale. b Heatmap showing the mRNA expression of genes in the TGF-β signaling pathway in mouse spinal cord from NanoString nCounter analysis. The gradient color represents the z-score of each gene across different samples, with red indicating higher expression and blue indicating lower expression of the gene across samples. c Bubble plot showing the mRNA expression of genes in the TGF-β signaling pathway in human spinal cord of subjects with varied ages. Bubble size represents the percentage of cells expressing the gene, and color scale bar represents the normalized expression value. d qRT-PCR results showing the mRNA expression of genes in the TGF-β signaling pathway across microdissected DC, VC and grey matter (GM) regions in young (2-3 months) and older mice (6-8 months); n = 9 mice for GM and DC, and n = 8 mice for VC. e, f Flow cytometry validation of microglial surface markers was conducted to compare 2-3-month-old (YM) and 12-13-month-old (OM) mice, including quantification (f) of the frequency of marker-positive cells within the total microglia population; n = 6 mice. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001. Multiple Mann-Whitney tests with FDR correction were used for comparisons between the age groups within each region in (d), and Kruskal-Wallis tests with Dunn’s post-hoc test were used for cross-region comparisons within each age group; two-tailed Mann-Whitney test or unpaired t-test was used in (f).
