Extended Data Fig. 6: iDISCO tissue clearing and wholemount imaging of spinal cord segments.
From: TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration
a A 3D view of spinal cord segments of 12-month-old Tgfbr2fl/fl (WT) and Cx3cr1CreER:Tgfbr2fl/fl (KO) mice (D25). Upper panel: whole 3D images and randomly selected slices showing Iba1 (in red, with expression strength indicated using a heatmap pseudo-colouring; left panel), MBP (green; middle panel) and merged (right panel) staining in the spinal cord. Scale bar=400μm. Bottom panel: corresponding magnified images in the DC, GM, and VC. Scale bar=150μm. b 3D reconstructed and segmented comparison in the DC of WT and KO mice. White scale bar=400μm; yellow scale bar=150μm. Representative images are from two independent experiments with similar results.
