Extended Data Fig. 7: Transcriptomic analysis of microdissected microglia from different spinal cord regions following Tgfbr2 deletion.
From: TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration
a Different spinal cord regions of Cx3cr1CreER:Tgfbr2fl/fl mice at 28 days post-tamoxifen administration were microdissected and microglia from the DC, VC and GM were sorted for RNA-seq using FACS. b A total of 91 differentially expressed genes (DEGs) were revealed when comparing microglia from the DC with microglia from the VC and GM (Log2 fold change≥1, q-value < 0.05; DESeq2). The Gene Ontology Biological Process (GO_BP) enrichment analyses show the enriched terms of the 30 upregulated genes in the DC. c The 30 upregulated genes in the DC were analyzed using Cytohubba with the MCC algorithm, and the hub genes along with their interactions were presented. The colored bubbles represent the top 5 hub genes, in descending order from red, through orange, to yellow. d Heatmap displaying the relative expression of genes revealed in (c) across the GM, VC and DC regions. Schematic in a created with BioRender.com.
