Fig. 6: Microglial Tgfbr2 deficiency alters the landscape of oligodendrocyte lineage cells in the spinal cord.
From: TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degeneration
a, A t-SNE plot of oligodendrocyte lineage cells from 12-month-old female WT mice and KO mice at D10, D20 and D30 post-tamoxifen administration (downsampled to 5,000 cells per group per time point for presentation). b, A bubble plot showing the expression of signature genes across different subclusters. Bubble size indicates the percentage of cells expressing the gene in the subcluster and the color scale bar indicates the normalized expression of the gene. c, The kinetic fold change (compared to WT condition) of each subcluster in a over time. d, A volcano plot depicting the DEGs between MOL1–4 and MOL5/6 (log2 fold change (FC) >1, adjusted P value <0.00; two-sided Wilcoxon rank-sum test with Benjamini–Hochberg correction). e, A volcano plot depicting the DEGs between DA-MOL and other MOLs (log2 fold change >1, adjusted P value <0.001; two-sided Wilcoxon rank-sum test with Benjamini–Hochberg correction). f,g, Spatiotemporal validation of DA-MOL in the spinal cord by staining of CC-1 (green), Serpina3n (magenta), fluoromyelin (white) and nuclei (Hoechst, blue) (f), with the inset showing the magnified image in the DC of KO_D20 mice, and quantification of Serpina3n MFI (g). Scale bar, 50 μm or 20 μm; n = 3, 5 and 4 mice for each group. Data are presented as mean ± s.e.m. *P < 0.05 and **P < 0.01; multiple paired t-tests with FDR correction.
