Extended Data Fig. 2: Characterization of neural differentiation growth-restricting genes (NGGs).

a, Dynamics of the relative abundance (mean counts across four sgRNAs) for five genes that are essential at day 4 but become growth-restricting by day 10. Error bars represent the standard error of the mean across the four sgRNAs. b–h, Clusters of protein–protein interactions (PPIs) among neural differentiation growth-restricting genes (NGGs) are shown. Only clusters with at least three proteins are presented. P represents the PPI enrichment p-value, which is based on the observed number of interactions versus expectation from a set of proteins of the same size randomly drawn from the genome. The colored outer part of the circle indicates the GO term associated with the gene, as described directly beneath the cluster. i, The top five Human Phenotype Ontology (HPO) terms enriched with NGGs.