Extended Data Fig. 6: Additional data characterizing the effect of OFC inactivation.
From: Orbitofrontal cortex drives predictive filtering of sensory responses
a-b, Additional data from A1 L2/3 pyramidal cell imaging during muscimol infusion into the OFCvl. a, Fraction of significantly excited (left) and suppressed (right) cells over six days. n = 7 mice, 1,177 responsive cells. Excited: *P = 0.031. Suppressed: *P = 0.016. Two-sided Wilcoxon signed-rank test. b, Same as a but for PBS infusion into the OFCvl. n = 3 mice, 439 responsive cells. Excited: P = 0.25. Suppressed: P = 1.0. c, Projection of sound-evoked A1 ensemble trial-to-trial activity dynamics onto Modewithin (left) and Modeacross (right) defined using data from Days 1–5. Data points represent individual trials (100 trials × 6 days) before and after muscimol infusion. The ensemble activity pattern shows partial recovery along Modeacross but not Modewithin following muscimol infusion. n = 7 mice, 734 cells imaged throughout the six days. d, Cosine similarity between Modewithin and Modeacross, indicating a nearly orthogonal relationship. e, Left: Change in sound-evoked ensemble activity along Modewithin between Trial 1 and Trial 10, illustrating prominent within-day, across-trial habituation around tone onset. Right: Change in sound-evoked ensemble activity along Modeacross from Day 1 to Day 5 (green) and from Day 5 to Day 6 (red). Both across-day habituation and its recovery by muscimol slowly ramp up during the sustained tone. f, Summary data showing the Ramp-up Index for across-day plasticity (day 1 to day 5), muscimol-induced recovery (Day 5 to Day 6), and within-day plasticity (Trial 1 to Trial 10). g, Change index for inhibitory responses across blocks of trials during muscimol infusion on Day 1 in naïve mice. Each data point represents a block of 25 trials. Red: muscimol (n = 4 mice). Dark gray: PBS control (n = 6 mice). h, Change index for inhibitory responses in individual neurons in blocks 6–8 compared to blocks 2–4 across all mice. n = 894 and 492 significantly suppressed neurons for PBS and muscimol. P = 0.55 (two-sided Wilcoxon rank-sum test). i, Change index for inhibitory responses in SST, VIP, and PV neurons comparing Day 5 and Day 6. SST, n = 51 responses from 8 mice, P = 0.30; VIP, n = 197 responses from 5 mice, ****P = 3.9 × 10−28; PV, n = 150 responses from 5 mice, ****P = 5.2 × 10−12 (two-sided Wilcoxon signed-rank test). Error bars and shades represent mean ± s.e.m.
