Fig. 6: NMDA receptors in A1 and SST cells are required for habituation.
From: Orbitofrontal cortex drives predictive filtering of sensory responses
a, Schematic illustrating the viral strategy to knock out NMDA receptors in A1 neurons (top). Representative two-photon image of A1 L2/3 neurons from a Grin1flox/flox mouse expressing GCaMP6s (green) and Cre (red) (bottom). b, Change index for excitatory (solid line) and inhibitory (dotted line) sound-evoked responses across days in Grin1flox/flox (red) and control (black) mice. Grin1flox/flox, n = 4 mice, 905 cells; control, n = 5 mice, 1,708 cells. c,d, Same as a,b but for knockout of NMDA receptors selectively in SST neurons. d, SST-Cre × Grin1flox/flox, n = 5 mice, 1,364 cells; control, n = 6 mice, 1,740 cells. e,f, Same as a,b but for knockout of NMDA receptors selectively in VIP neurons. f, VIP-Cre × Grin1flox/flox, n = 4 mice, 996 cells; control, n = 5 mice, 1,494 cells. g, Summary scatter-plots showing the change index of excitatory responses in individual neurons on day 5 compared to day 1. Grin1flox/flox, Cre− control (Ctrl), n = 229 excited cells. Grin1flox/flox + AAV-Cre (KOA1), n = 220. SST-Cre × Grin1flox/flox (KOSST), n = 262. VIP-Cre × Grin1flox/flox (KOVIP), n = 255. Ctrl versus KOA1, ****P = 3.6 × 10−5. Ctrl versus KOSST, ****P = 1.8 × 10−5. Ctrl versus KOVIP, P = 1.0. KOA1 versus KOSST, P = 1.0. KOA1 versus KOVIP, **P = 0.0019. KOSST versus KOVIP, **P = 0.0020 (two-sided Wilcoxon rank-sum test with Bonferroni correction). h, Summary diagrams of sensory habituation pathways. Error bars and shades represent mean ± s.e.m.
