Extended Data Fig. 2: Additional data related to females and core body temperature measurements.
From: Cortical regulation of collective social dynamics during environmental challenge
a. Schematics illustrating 5 unique group states derived via automated SLEAP pose estimation and identity tracking, ranging from most dispersed to most aggregated. b. Frequency of group states observed at 20 °C, 15 °C, 10 °C, or 5 °C during thermal challenge assay in females (n = 6 groups of 4 individuals). c. Mean group state duration in seconds observed at 20 °C, 15 °C, 10 °C, or 5 °C during thermal challenge assay in females (n = 6 groups of 4 individuals). d. Schematic illustrating behavioral thermotaxis assay. Animals are placed in a behavioral chamber at 5 °C with free access to a 30 °C warm corner. e. Comparison of percent time spent in warm corner during thermotaxis assay in males and females (n = 8 males, 8 females). f. Schematic measurement of core body temperature in pairs vs groups. g. Average change in core body temperature (°C) in pair and group conditions (n = 12 animals). h. Area under the curve (°C) from 5-30 min for pair and group conditions (n = 12 animals). i. Area under the curve (°C) during first and last minute of session for pair and group conditions (n = 12 animals). j. Comparison of total locomotion (cm) when animals are exposed to thermal challenge alone or in groups (n = 12 animals). k,l. Correlation between total locomotion and change in core body temperature in alone (k) and group (l) conditions (n = 12 animals). Box plots: center line–median; box limits–upper/lower quartiles; whiskers–min/max. Statistical tests: two-way ANOVA with Bonferroni post-hoc (b,c,i), two-tailed Wilcoxon matched pairs tests (e,h,j), and linear regressions (k,l). *P < .05, **P < .01, ***P < .001, ****P < .0001. See Supplementary Table 1 for statistical details.
