Extended Data Fig. 4: Template-matching algorithm used to track the brain is robust across the field of view.
From: Brain motion is driven by mechanical coupling with the abdomen
a. An image of the GFP-expressing parenchyma. Each of the eight bounding boxes (white) represents a tracking template area for the matching algorithm to follow. b. The targets were tracked at each of the eight locations and the mean and 90 percent confidence interval (shading) were calculated and plotted. N = 1 recording location in one mouse. The tight confidence interval bounds highlight the confidence in tracking different structures at various locations within the image as well as a lack of brain distortion within the field of view, indicating rigid translation. Frame rate was 106.89 frames/sec. Note the lack of appreciable motion at the cardiac or respiratory frequencies. c. Images of the brain (from a) when the mouse is at rest (top) and during a locomotion event (bottom). The neuron seen in the bounding box (gray) displaces rostrally and laterally during locomotion when compared to its resting position.
