Extended Data Fig. 6: Asymmetric and flexible configuration of CA in helical assemblies. | Nature Structural & Molecular Biology

Extended Data Fig. 6: Asymmetric and flexible configuration of CA in helical assemblies.

From: Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A

Extended Data Fig. 6

a, Distances between opposing CACTD domains in CA hexamer (measured between Cα of N195) from helical symmetry (-13, 8). b, Correlation plot of distances between CACTD domains versus hexamer array angles from five different helical assemblies. c, d, Close-up views of asymmetric β-hairpins (c, orange) and Arg18 residues adopting two conformations at the hexamer center (d). e, Overlay of electron density map with atomic model, showing two Arg18 side chain positions. f, Overlay of three Arg18 side chains in CA tubular structure (gold) with those in crystal structures in the absence of IP6 (blue, PDB 4WYM, PDB 4XFX, PDB 4XFY, PDB 4XFZ, PDB 5HGL). g, Overlay of Arg18 in crystal structures in complex with IP6 (PDB 6bht), same view as in f. 12 CA molecules from two hexamers (colored in pink and cyan, respectively) in one asymmetric unit were aligned on CANTD.

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