Extended Data Fig. 10: Semi-quantitative analysis of CypA levels in primary CD4+ T cells. | Nature Structural & Molecular Biology

Extended Data Fig. 10: Semi-quantitative analysis of CypA levels in primary CD4+ T cells.

From: Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A

Extended Data Fig. 10

a, CD4+ T cell CypA levels from two different blood donors (labeled D1 and D2) were compared by immunoblotting versus a dilution series of purified recombinant CypA protein. Where indicated, cells were cultured with 5 μg/ml of phytohaemagglutinin (+PHA) for 48 h prior to lysis. Lamin B1 was monitored as a loading control. Approximate ng amount of Cyclophilin A in each of the last four lanes is marked in red color. b, Signal intensity of each band in the dilution series in A was plotted against protein amount to generate a standard curve. Uncropped blot images are available as source data.

Source data

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