Extended Data Fig. 3: 3D reconstructions of CA hexamer from five helical symmetries.

a, Electron density maps of CA tubes in the absence and presence of CypA and with different helical symmetries, as indicated. b, Fourier-shell-correlation (FSC) plots of CA hexamer density maps reconstructed from five helical symmetries. The highest resolution at FSC=0.143 is 3.6 Å from (-13, 8) symmetry. c, The FSC plot between the refined CA hexamer model and the 3.6 Å cryoEM density map from (-13, 8) symmetry. d, Representative density map from (-13, 8) symmetry overlapped with the refined atomic model, shown are the helices between adjacent CA monomers in different color. The map was contoured at 2𝜎.