Extended Data Fig. 9: Identification of lipids copurified with CaChs2 by LC/MS.
From: Structural basis for inhibition and regulation of a chitin synthase from Candida albicans
a, Total ion chromatogram (TIC) of normal phase LC/MS of lipids extracted from the CaChs2 protein sample. b, Representative chemical structure of ceramide and mass spectrum of the 2.88 min TIC peak in a showing the chloride adduct [M + Cl]- ions of ceramide species. c, Representative chemical structure of and mass spectrum of the 16.20 min TIC peak in a showing the [M-H]- ions of PE molecular species. This identification of ceramide and PE was supported by exact mass measurement and MS/MS. The TIC peaks at 14.09, 21.16 and 27.18 min are consistent with glycodiosgenins (GDN) containing two, three and four sugars whose [M + Cl]- ions are observed at m/z 875.5, 1037.5 and 1199.5, respectively.
