Extended Data Fig. 3: H4S47 O-GlcNAcylation does not affect cell viability.
From: H4S47 O-GlcNAcylation regulates the activation of mammalian replication origins
a, b, Analysis of cell survival and apoptosis. HEK293T cells were transfected and treated as in Extended Data Fig. 2a. Etoposide (10 μM) treatment for 24 h was used as a positive control. Following staining with Annexin V and PI, cells were analyzed by flow cytometry. Representative FACS profiles (a) and quantifications of apoptotic cells (b, left) and viable cells (b, right) are demonstrated. n = 3 biologically independent experiments. P values were calculated by Tukey’s multiple comparison test (two-way ANOVA), means ± s.d., n.s., non-significant.
