Extended Data Fig. 7: Confirmation of the regulation of MCM complex by H4S47 O-GlcNAcylation.
From: H4S47 O-GlcNAcylation regulates the activation of mammalian replication origins
a, b, Assay for subunits in MCM complex associating with H4. HT1080 (a) or A549 (b) cells were transfected and treated as in Fig. 1a. IgG served as a negative control. Representative experiment (left) and quantification of MCM2/6 levels were shown (right). n = 3 (a), n = 4 (b) biologically independent experiments. P values were calculated by Tukey’s multiple comparison test (two-way ANOVA), means ± s.d., n.s., non-significant. c, d, Examination of MCM2 pS53 in chromatin fraction. HT1080 (c) or A549 (d) cells were transfected and treated as in Fig. 1a. Chromatin pellets were assayed with indicated antibodies. Representative experiment (left) and quantification of MCM2 pS53 levels were shown (right). n = 3 biologically independent experiments. P values were measured by paired, two-tailed t-tests, means ± s.d., n.s., non-significant. a.u., arbitrary unit.
