Extended Data Fig. 3: Introduce fluorescence protein genes to endogenous locus generated functional fusion proteins.
a, WT, ddx-19(-) and ddx-19::gfp animals were grown on dpy-11 RNAi, F1 progeny were grown on OP50. The percentage of Dumpy animals in inheriting F1 was scored at L4 larval stage. n = 4 biological replicates. Error bar, ±s.d. Student’s t-test, two-tailed. b, WT, npp-14(-), npp-14::mCherry::ha and npp-14::gfp::3xflag animals were grown at 25 °C, brood size was counted at indicated generations. n = 3 biological replicates. Error bar, ±s.d. c, WT, glel-1(-) and ha::mCherry::glel-1 animals were subjected to dpy-11 RNAi treatment, percentage of Dpy animals were scored at F1 generation. n = 3 biological replicates. Error bar, ±s.d. d-e, WT, mut-2(-) and mut-2::mCherry animals (d) or WT, rsd-2(-) and rsd-2::gfp animals (e) were treated with pos-1 RNAi. The percentage of hatched animals was scored. n = 3 biological replicates. Error bar, ±s.d. Student’s t-test, two-tailed. Source numerical data are available.
