Extended Data Fig. 6: The localization interdependence of DDX-19, NPP-14 and GLEL-1. | Nature Structural & Molecular Biology

Extended Data Fig. 6: The localization interdependence of DDX-19, NPP-14 and GLEL-1.

From: A nuclear pore-anchored condensate enables germ granule organization and transgenerational epigenetic inheritance

Extended Data Fig. 6: The localization interdependence of DDX-19, NPP-14 and GLEL-1.

a, Fluorescence imaging of embryos expressing DDX-19::GFP in npp-14 mutants background. Scale bar, 5 μm. n > 3 animals. b-c, Fluorescence imaging of WT(b) and npp-14(-) (c) animals expressing DDX-19::GFP and PGL-1::TagRFP. n > 3 animals. d, Fluorescence imaging of animals expressing NPP-14::mCherry. Scale bar, 10 μm. White arrows indicate NPP-14::mCherry in somatic cells. n > 3 animals. e, Fluorescence imaging of animals expressing mCherry::GLEL-1. Scale bar, 10 μm. White arrows indicate mCherry::GLEL-1 in somatic cells. n > 3 animals. f, A schematic diagram of the C. elegans tissues nuclear pore. g, Spinning disc confocal imaging of animals expressing indicated proteins. Scale bar, 1 μm. n > 3 animals. h, Fluorescence imaging of WT, ddx-19(-), npp-14(-), and glel-1(-) animals expressing NPP-21::GFP, NPP-1::GFP, or TagRFP::NPP-9. Scale bar, 5 μm. n > 3 animals. i, Fluorescence imaging of animals expressing NPP-14::mCherry in WT or ddx-19(-) background. Scale bar, 5 μm. n > 3 animals. j, Fluorescence imaging of animals expressing NPP-14::mCherry in WT or glel-1(-) background. Scale bar, 5 μm. n > 3 animals.

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