Extended Data Fig. 8: Increased mixing of P and Z compartment components after loss of DDX-19, NPP-14, and GLEL-1 related to Fig. 6.

a-b, Fluorescence imaging of animals expressing GFP::ZNFX-1 and PGL-1::TagRFP in WT, ddx-19(-), npp-14(-), and glel-1(-) background (a). Image J analysis to measure the degree of colocalization between GFP::ZNFX-1 and PGL-1::TagRFP in WT and mutant animals (b). Scale bar, 1 μm for cells, 0.5 μm for inset. n = 27 (3 animals per biological replication, 3 nuclei per animal, 3 granules per nuclei). Error bar, ±s.d. Student’s t-test, two-tailed. c-d, Fluorescence imaging of animals expressing MUT-16::GFP and PGL-1::TagRFP (c) or MUT-16::GFP and TagRFP::ZNFX-1(d) in WT, ddx-19(-), npp-14(-) and glel-1(-) background. n > 3 animals. e, Fluorescence imaging of PGL-1::mCardinal, TagRFP::ZNFX-1, and MUT-16::GFP in WT, ddx-19(-), npp-14(-) and glel-1(-) animals. Scale bar, 1 μm for cells, 0.5 μm for inset. n > 3 animals. f, Quantification of the degree of colocalization in (e). n = 27 (3 animals, 3 nuclei per animal, 3 granules per nuclei). Error bar, ±s.d. Student’s t-test, two-tailed. g, ddx-19, npp-14 or glel-1 deletion were generated in animals expressing PGL-1::mCardinal, TagRFP::ZNFX-1 and MUT-16::GFP using CRISPR/Cas9. Fluorescence imaging was performed on animals expressing indicated proteins immediately at F2 generation when the heterozygous deletions became homozygous. n > 3 animals. h, Fluorescence imaging of animals expressing DDX-19::GFP and MUT-2::mCherry in WT, npp-14(-), and glel-1(-) background. n > 3 animals. i, Quantification of sphericity of DDX-19::GFP marked D compartment, PGL-1::mCardinal marked P compartment, and TagRFP::ZNFX-1 marked Z compartment in WT animals expressing DDX-19::GFP, PGL-1::mCardinal, and TagRFP::ZNFX-1. n = 27 (3 animals, 9 granule per germline). Student’s t-test, two tailed. Error bar, ±s.d. Source numerical data are available.

Source data