Extended Data Fig. 5: Only Atg1, but not the PAS scaffold proteins, are transported to the vacuole for degradation.
From: Recruitment of Atg1 to the phagophore by Atg8 orchestrates autophagy machineries
(A-B) Subcellular distribution of 2xGFP tagged Atg1, Atg13, Atg17, Atg29, and Atg31 at 1 h (S1) and 4 h (S4) after starvation, in wild type (A) or pep4Δ (B) background cells. Only Atg1-2GFP showed prominent translocation into vacuoles at 4 h. Representative image slices are shown. Scale bar, 5 um. (C) The processing of GFP tagged proteins examined by immuno-blotting. Only Atg1-GFP showed prominent processing into free GFP at 4 h.
