Extended Data Fig. 3: DMS-MaPseq and targeted DMS probing reproducibility between replicates.
From: Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo
A) r2 between replicates for each intron in S. cerevisiae versus the average sequencing coverage between replicates. B) Bar graph comparing the number of high confidence stems that are shared vs discordant between replicates for introns in different ranges of replicate correlation. C) r2 between replicates of targeted DMS probing for 30 introns without (left) or with (right) heat denaturation. D) The number of high confidence stems found in 24 introns with r2 > 0.9 from targeted DMS probing and r2 > 0.6 from DMS-MaPseq. E) The number of high confidence stems found in 22 introns with r2 > 0.9 from targeted DMS probing of denatured RNA and r2 > 0.6 from DMS-MaPseq.
