Extended Data Fig. 5: Secondary structure features for introns and non-splicing decoy sequences.
From: Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo
A) Intron and decoy sequence schematic. Nucleotide lengths depicted are representative lengths for introns and decoy sequences, with decoy sequences chosen to have 5’ splice site, branch point, and 3’ splice site placements matching length distributions from canonical introns (see Methods). B) Secondary structure features are enriched in standard canonical spliced introns in yeast, but not in decoy sequences (genomic intervals which match splice site sequences and yet do not splice). *p-value < 0.01 by two-sided Wilcoxon ranked-sum test. Left: N = 140 canonical introns were compared to controls, yielding p-values (left to right): <1E-4, 0.00027, 0.00072, <1E-4, <1E-4. Right: N = 167 decoy introns were compared to controls, yielding p-values (left to right): 0.74, 0.23, 0.68, 0.37, 0.10. Embedded box plots mark the median as the center white point and include a box from the 25th (Q1) to 75th (Q3) percentile, extending whiskers to the smallest and largest value that fall within 1.5 times the interquartile range below Q1 and above Q3.
