Extended Data Fig. 8: Structure variant library design to measure spliced and unspliced RNA levels for intron variants.
From: Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo
A) Computational pipeline for designing variant and rescue sequences to assess stem and loop sets in an intron. B) Sample base-pair probability matrix comparison between a set of wildtype (left), variant (middle), and rescue (right) sequences. The stem set targeted by this variant and rescue sequence are bracketed. C) Constructs used for constructing the landing pad strain LPL001 (top) and for transforming the intron library via genomic integration into LPL001 (bottom). D) Based on simulations sampling 10,000 or 50,000 random barcodes, the number of barcodes within 2, 3, or 4 edit distance of another barcode. The vertical line indicates the chosen barcode length (12).
