Extended Data Fig. 7: CTCF and cohesin-associated factors in single-Stag GSCs.
From: The mitotic STAG3–cohesin complex shapes male germline nucleome
a, Upset plot for overlap of CTCF-binding sites among WT and single-Stag GSCs. b, Comparison of normalized CTCF intensity in CTCF/cohesin co-occupied sites between WT and single-Stag GSCs as indicated. c, Spike-in-normalized RAD21 enrichment at CTCF-binding sites in WT and single-Stag GSCs. d, The numbers of TAD boundaries defined by the TAD boundary strength measured by cooltools152 in the indicated single-Stag GSCs. e, Violin and box plots of the loop strength quantified by Chromosight84. Box plots (45,701 loops each) are as in Fig. 2d. f, Representative image of Western blot analysis for the enrichment/expression of PDS5A/B and RAD21 in WT and single-Stag GSCs in the chromatin fraction. Two technical replicates were analyzed. g, Abundance of acetylated SMC3 (SMC3ac), PDS5A/B and RAD21 in the chromatin fraction as quantified by Western blot analysis as in (f) normalized by histone H3. Two technical replicates were analyzed. Histone H3, used as a loading control, was obtained from three different lanes. All proteins were derived from the same protein lysate.
