Extended Data Fig. 1: The in vitro system recapitulates key features of germ-cell development.
From: The mitotic STAG3–cohesin complex shapes male germline nucleome
a, Correlation heatmap of transcriptomic profiles of the top 5,000 most variable genes among the indicated cell types [ESCs; EpiLCs; d2PGCLCs; d4c7PGCLCs; GSCs31,41,146; E11.5/12.5 germ cells38; and P7 Kit− spermatogonia]. b, Correlation heatmap of 5mC profiles in the genome-wide 2 kb bins among the indicated cell types: E13.5 germ cells (ESCs; EpiLCs; d2PGCLCs; d4c7PGCLCs; GSCs31,41,147; E13.5 germ cells148; and P7 Kit− spermatogonia24). c, Unsupervised hierarchical clustering (UHC) based on Euclidean distance among 100 kb compartment score tracks of the indicated cell types [in vitro (ESCs/EpiLCs/d4c7PGCLCs/GSCs)19, in vivo [E3.5 ICM/E6.5 epiblast cells149/E11.5 PGCs20], and spermatogonia in several publications81,150,151. Note that spermatogonia reported by Zuo et al.81 and Vara et al.150 were purified by FACS based on DNA content and include undifferentiated and differentiating spermatogonia, while those by Luo et al.151 were collected with unit gravity sedimentation followed by purity confirmation with immunofluorescence (IF) analysis. Primitive type A spermatogonia (priSG-A) expressed GFRA1 and were enriched for spermatogonial stem cells, while type A spermatogonia expressed KIT and were enriched for differentiating spermatogonia151.
