Extended Data Fig. 9: Quality of the SUMO E1-UBC9-SUMO1(t)/SUMO1(a) cryo-EM reconstruction.
From: Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer
a, Schematic representation of SUMO E1-E2 thioester transfer reaction. b, SDS-PAGE of sample preparation steps of the double-loaded SUMO E1-E2 complex used for cryo-EM analysis (right). c, The final Euler angle distribution of particles used for 3D refinement. d, Fourier Shell Correlation (FSC) plot from two half maps, with an overall resolution of 3.6 Å as determined at 0.143 criterion. e, Local resolution map color-coded from lower (red) to higher resolution (blue). f, Overall map (left) and detailed views of local cryo-EM density for SUMO E1, UBC9, SUMO1(a), SUMO1(t), AMP and SUMO E1-UBC9/SUMO1(t) active site regions of the SUMO E1 complex. g, Cryo-EM map of the SUMO E1-UBC9/SUMO1(a)/SUMO1(t) complex with two views related by a 180° rotation about the y-axis (left). Maps of the individual subunits are shown on the right. h, Superimposition of SUMO E1 single loaded (grey) onto SUMO E1 double loaded.
