Extended Data Fig. 1: Quality of the SUMO E1-UBC9/SUMO1(a) complex cryo-EM reconstruction.
From: Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer
a, The final Euler angle distribution of particles used for 3D refinement. b, Fourier Shell Correlation (FSC) plot from two half maps, with an overall resolution of 2.7 Å as determined at 0.143 criterion. c, Local resolution map color-coded from lower (red) to higher resolution (blue). d, Overall map (left) and detailed views of local cryo-EM density for IAD, AAD, UBC9, SUMO1 C-terminal AMP adenylate and E1-E2 disulphide regions of the complex. e, Cryo-EM map of the SUMO E1-UBC9/SUMO1(a) complex with two views related by a 180° rotation about the y-axis (left). Maps of the individual subunits are shown in the right with IAD (slate), AAD (lightpink), FCCH (forestgreen), SCCH (purple), UFD (orange), Cys cap (magenta), UBC9 (cyan) and SUMO1(a)(yellow).
