Extended Data Fig. 4: Model overlays of SZT2 and C12orf66.
From: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
a, Overlay of SZT2SZ1 (light gray) with spermine synthase (light sky blue, PDB 3C6K), SZT2NTD, Depdc5NTD-SABA domains (forest green, PDB 7T3B), and CD11a I domain (rosy brown, PDB 1LFA). RMSD values for the superimposed structures are shown in Supplementary Table 2. b, Overlay of C12orf66NTD (orange) with BroxBro1 (cornflower blue, PDB 3R9M), SRP68 (pink, PDB 7QWQ), C12orf66roadblock (orange), and Lamtor2 (purple, PDB 6B9X). RMSD values for the superimposed structures are shown in Supplementary Table 2. c, Close-up view of the SZT2-C12orf66 interface. W3428 of SZT2 is required to interact with C12orf66 L216 and F269, as shown by the pulldown assay in d-e. d, Pulldown experiment of wild-type or mutated SZT2 with KPTN, ITFG2, and C12orf66. TSF, twin-strep-flag. e, Pulldown experiment of wild-type or mutated C12orf66 with KPTN, ITFG2, and SZT2. TSF, twin-strep-flag. Data in d and e are representative of three independent experiments.
