Extended Data Fig. 5: Cryo-EM structure determination of the human GATOR1-KICSTOR supercomplex.
From: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
a, EM image of the negatively stained GATOR1-KICSTOR supercomplex. The shape of the supercomplex can be recognized and circled in the raw image. Data in a is representative of at least three independent experiments. b, Representative motion-corrected cryo-EM micrograph (of 9,936 micrographs from 1 dataset) of the human GATOR1-KICSTOR complex. c, Representative 2D class averages for the GATOR1-KICSTOR supercomplex. d, Workflow for the cryo-EM data processing of human GATOR1-KICSTOR complex. NU-refinement: non-uniform refinement. e-g, FSC plots (e), angular distribution heatmap (f) and local resolution estimation (g) for the consensus reconstruction of GATOR1-KICSTOR supercomplex. h-j, Masking and local refinement results of Nprl3-SZT2. Half-map FSC plots (h), angular particle distributions (i) and local resolution estimation (j) from local refinement on Nprl3-SZT2 regions. k, Composite map of GATOR1-KICSTOR supercomplex was generated for subsequent model building.
