Extended Data Fig. 10: Hypothetical model of KICSTOR-GATOR1-GATOR2-Rag-Ragulator complex tethered on the lysosomal membrane.
From: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
a, Model was assembled using the reported structures of GATOR2 (PDB 7UHY), an AlphaFold2 prediction for WDR59 protein, and dual mode of GATOR1 which is bound to two copies of Rag-Ragulator, one in the “GAP conformation” and another one in the “inhibitory conformation” (PDB 7T3C) and KICSTOR from this study. b, Color code for the KICSTOR1, GATOR1, Rag-Ragulator and GATOR2 subunits. c, The reported structures of GATOR2 (PDB 7UHY), dual mode of GATOR1, which is bound to two copies of Rag-Ragulator, one in the “GAP conformation” and another one in the “inhibitory conformation” (PDB 7T3C), and an AlphaFold2 prediction for WDR59 protein, were assembled and superimposed on SEA complex (PDB 8ADL). The dotted line box indicates the interface between GATOR1 and GATOR2. d, KICSTOR from this study was superimposed on the GATOR1-dual mode. The dotted line box indicates the interface between GATOR1 and KICSTOR. e, Zoomed-in view on the clashing of SZT2SZN1-SZN2 and Sec13.
