Extended Data Fig. 1: Cryo-EM structure determination of the full-length KICSTOR complex.
From: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
a, Representative motion-corrected cryo-EM micrograph (of 30,943 micrographs from 4 datasets) of the full-length human KICSTOR complex. b, Representative 2D class averages of the full-length KICSTOR complex. c, Workflow for cryo-EM data processing of full-length KICSTOR datasets. NU-refinement: non-uniform refinement. d, FSC plots for the consensus reconstruction of the full-length KICSTOR in state 1 are between two independently refined half-maps with no mask (blue), spherical mask (orange), loose mask (green), and tight mask (red). A cutoff value of 0.143 was used to estimate the resolution. e, Angular particle distribution for projection views of full-length KICSTOR in state 1, calculated using CryoSPARC. The heat map shows the number of particles for each viewing angle. f, Full-length KICSTOR in state 1 density map, color-coded according to local resolution estimation. g-i, Results of NU-refinement for the consensus reconstruction of full-length KICSTOR in state 2. (g) FSC curves, (h) angular distribution heatmap, (i) local resolution estimation, with the map color-coded to reflect the resolution. j, Masking and local refinement applied to the SZT2SZ1-SZ2-SZ3 and SZT2SZ7-C12orf66 regions of full-length KICSTOR in state 1. k, Masking and local refinement applied to the SZT2SZ1-SZ2-SZ3 or SZT2SZ7-C12orf66 regions of full-length KICSTOR in state 2.
