Extended Data Fig. 2: Overview of cryo-EM processing of the KICSTOR C-terminal core complex (CCC).
From: Architecture of the human KICSTOR and GATOR1–KICSTOR complexes
a, Superose 6 size-exclusion chromatogram profile of the human KICSTOR CCC. The peak corresponding to KICSTOR CCC is marked with a black asterisk. Coomassie blue-stained SDS-PAGE analysis of the purified KICSTOR. MW, molecular weight. mAU, milliabsorbance. Data in a is representative of at least three independent experiments. b, Representative motion-corrected cryo-EM micrograph (of 8,829 micrographs from 1 dataset) of the KICSTOR C-terminal core complex. c, Representative 2D class averages of the KICSTOR CCC. d, Workflow for cryo-EM data processing of the KICSTOR CCC. NU-refinement: non-uniform refinement. e-g. Half-map FSC plots, angular particle distributions and local resolution estimation from consensus reconstructions of the KICSTOR CCC for state 3 (class 1), state 4 (class 2), and state 5 (class 3). h-i. Masking and local refinement applied to the SZT2SZ7-C12orf66 regions of the KICSTOR CCC for state 3 and state 5. j, Individual composite maps generated for different states of the KICSTOR CCC, prepared for subsequent model building.
