Extended Data Fig. 6: Phylogenetic and structural analysis of PtuB.
From: Structural basis for retron co-option of anti-phage ATPase-nuclease
a. Phylogenetic analysis of both retron Type I-A (black) and Septu (orange) PtuB sequences. b. Close-up view of the C-terminal domain of PtuB (CTD) interacting with a pair C-terminal extension (CTE) of PtuA. c. Superimposed PtuB structures between the Ec78 (beige) and the Septu (gray, PDB code: 8EE7) systems. The catalytic site is marked by a red dot. The region displaying the largest difference is marked by Ec78 PtuB residues. d. Comparison of the HNH domain active site among Ec78 PtuB, Septu PtuB (PDB code: 8EE7) and the Cas9 from Acidothermus cellulolyticus (PDB code: 8D2K). e. Gel filtration profiles of free PtuA and PtuB in comparison with that of PtuA(Q361L)B complex and caliberated molecular weight standards. The estimated molecular weight markers are marked. The eluted fractions for each run were verified for the presence of the corresponding proteins by SDS-PAGE (lower panel). Protein markers are Color Prestained Protein Standard, Broad Range (10-250 kDa) (New England BioLab).
