Fig. 3: Structure overview of the Ec78 retron and analysis on ncRNA and msDNA processing.
From: Structural basis for retron co-option of anti-phage ATPase-nuclease
a, The secondary and tertiary structures of the ncRNA and msDNA observed in the Ec78 structures. Only the RT is retained for simplicity with its characteristic domains labeled. b, Gel analysis results of the nucleic acids extracted from the purified Ec78, RT–ncRNA and RT-R143A K146A–ncRNA (RT(RK)) (right) or from the RNase-A-treated cell extract (left). RNA markers are the Low Range ssRNA Ladder (New England Biolab). c, Density maps (top) and cartoon representations (bottom) of the Ec78 retron complex. The 4:2:1 complex is shown to the left with one PtuB in trans with respect to the top RT, and the 4:2:2 complex is shown to the right with the corresponding PtuB in cis with respect to the top RT. Orange dots indicate the bound ADP molecules. Insets show the close-up views of the density around the two selected ADP molecules in the 4:2:1 complex. Experiments in b were repeated independently with similar results (Source Data Fig. 3).
