Extended Data Fig. 4: Cryo-EM structure of TOD4 complexed with dsDNA substrate.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a, Cryo-EM structure of TOD4inact complexed with dsDNA substrate (colored) is in close agreement with the design model (gray). Schematic diagram (top) shows the domain organization of TOD4, comprising TALE, orienting domain C4 (candidate 4), and Ddd_Ss_S deaminase. b, The zoomed view showing the last TALE repeat (purple), orienting domain C4 (blue) and inactive Ddd_Ss_S (pink) in TOD4 interacting with dsDNA substrate (orange). c, d, Detailed views showing that residues from the orienting domain C4 form extensive interactions with both the last TALE repeat and the Ddd_Ss_S deaminase. e, Cryo-EM structures of Ddd_Ss_S in TOD6inact and TOD4inact complexed with dsDNA substrate are almost identical.
