Extended Data Fig. 6: On-target and off-target editing activities of mDdCBE-TOD6.
From: Computational design of a high-precision mitochondrial DNA cytosine base editor
a–f, Average C•G to T•A editing efficiencies at on-target (red dots, designated target site) and off-target (gray dots) sites across mtDNA for MT-CYB-mDdCBE (a), MT-CYB-mDdCBE-TOD6 (b), MT-ND1.1-mDdCBE (c), MT-ND1.1-mDdCBE-TOD6 (d), MT-ND3-mDdCBE (e) and MT-ND3-mDdCBE-TOD6 (f). Sites with average editing frequency greater than 1% are shown. Data are shown as means from n = 2 independent experiments. g, Venn diagram showing the overlap of off-target sites among MT-CYB-mDdCBE, MT-ND1.1-mDdCBE and MT-ND3-mDdCBE. h, Venn diagram showing the overlap of off-target sites among MT-CYB-mDdCBE-TOD6, MT-ND1.1-mDdCBE-TOD6 and MT-ND3-mDdCBE-TOD6. Source data are provided as a Source Data file.
